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广东快乐十分助手下载:Role for the shoot apical meristem in the specification of juvenile leaf identity in Arabidopsis
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Developmental transitions during shoot development in plants are regulated by factors originating outside and within the shoot apical meristem (SAM). The best-known example of this is the vegetative-to-reproductive transition, which is initiated by a leaf-derived signal that transforms the vegetative SAM into a developmentally stable inflorescence meristem. Although the juvenile-to-adult vegetative transition (vegetative phase change) is also thought to be regulated by factors exogenous and internal to the SAM, how this process is coordinated spatially remains unknown. Here we demonstrate that the SAM specifies leaf identity early in development, but that leaves become more important determinants of shoot identity as the shoot ages. We also reveal a role for the plant aging pathway in the regulation of meristem size.
The extent to which the shoot apical meristem (SAM) controls developmental decisions, rather than interpreting them, is a longstanding issue in plant development. Previous work suggests that vegetative phase change is regulated by signals intrinsic and extrinsic to the SAM, but the relative importance of these signals for this process is unknown. We investigated this question by examining the effect of meristem-deficient mutations on vegetative phase change and on the expression of key regulators of this process, miR156 and its targets, SPL transcription factors. We found that the precocious phenotypes of meristem-deficient mutants are a consequence of reduced miR156 accumulation. Tissue-specific manipulation of miR156 levels revealed that the SAM functions as an essential pool of miR156 early in shoot development, but that its effect on leaf identity declines with age. We also found that SPL genes control meristem size by repressing WUSCHEL expression via a novel genetic pathway.
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Author contributions: J.P.F. and R.S.P. designed research; J.P.F. performed research; J.P.F. contributed new reagents/analytic tools; J.P.F. and R.S.P. analyzed data; and J.P.F. and R.S.P. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1817853116/-/DCSupplemental.
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