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陕西快乐十分规则:DNA methylation analysis and editing in single mammalian oocytes
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Many mammalian nongenetic diseases and developmental disorders originate from oocyte DNA methylation abnormalities. However, prevention and correction of this maternally transmitted nongenetic disorder remains challenging because of the lack of strategy that can evaluate and manipulate specific methylation at single oocyte level. In this study, using several models of epigenetic inheritance via oocytes, we have shown that specific methylation in a single oocyte can be evaluated from its sibling PB1 and can be edited in a targeted manner. Our study provides a strategy for prevention and correction of maternally transmitted nongenetic diseases or disorders, and will also facilitate the investigation of maternally transmitted nongenetic information at the very beginning of life.
Mammalian oocytes carry specific nongenetic information, including DNA methylation to the next generation, which is important for development and disease. However, evaluation and manipulation of specific methylation for both functional analysis and therapeutic purposes remains challenging. Here, we demonstrate evaluation of specific methylation in single oocytes from its sibling first polar body (PB1) and manipulation of specific methylation in single oocytes by microinjection-mediated dCas9-based targeted methylation editing. We optimized a single-cell bisulfite sequencing approach with high efficiency and demonstrate that the PB1 carries similar methylation profiles at specific regions to its sibling oocyte. By bisulfite sequencing of a single PB1, the methylation information regarding agouti viable yellow (Avy)-related coat color, as well as imprinting linked parthenogenetic development competency, in a single oocyte can be efficiently evaluated. Microinjection-based dCas9-Tet/Dnmt–mediated methylation editing allows targeted manipulation of specific methylation in single oocytes. By targeted methylation editing, we were able to reverse Avy-related coat color, generate full-term development of bimaternal mice, and correct familial Angelman syndrome in a mouse model. Our work will facilitate the investigation of specific methylation events in oocytes and provides a strategy for prevention and correction of maternally transmitted nongenetic disease or disorders.
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Author contributions: Y.W., Y.D., and Y.S. designed research; Y.W., J.L., Q.Z., C.-R.Y., and Z.-A.Z. performed research; Z.-A.Z. and Y.Z. contributed new reagents/analytic tools; Y.W., C.-R.Y., and Z.-A.Z. analyzed data; and Y.W., C.-R.Y., and Y.S. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1817703116/-/DCSupplemental.
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